Biotechnology and Its Applications
Test 5

Correct answer Carries: 4.

Wrong Answer Carries: -1.

Which term refers to the fusion of protoplasts from different plant species to form a hybrid?

Tissue culture
Somatic hybridization
Micropropagation
Gene editing
2

Somatic hybridization involves the fusion of protoplasts from different plant species to produce hybrids.

What is the significance of transgenic mice in biotechnology?

Increased milk production
Disease modeling for human research
Production of biofuels
Crop improvement
2

Transgenic mice are widely used as models to study human diseases such as cancer and Alzheimer’s.

Which property allows a single plant cell to regenerate into a whole plant in tissue culture?

Pluripotency
Totipotency
Multipotency
Somatic hybridization
2

Totipotency is the ability of a single plant cell to regenerate into a complete plant under suitable conditions.

Which term refers to the use of biological resources without proper authorization?

Bioethics
Bioremediation
Biopiracy
Biosafety
3

Biopiracy refers to the use of biological resources without proper authorization or compensation.

Which enzyme is used to cut DNA at specific sequences in genetic engineering?

DNA ligase
Restriction enzyme
Reverse transcriptase
DNA polymerase
2

Restriction enzymes are used to cut DNA at specific sequences in genetic engineering processes.

Which term refers to the process of making DNA from an RNA template?

Transcription
Reverse transcription
Replication
Translation
2

Reverse transcription is the process of making complementary DNA (cDNA) from an RNA template.

Which genetically modified plant was developed for resistance to the stem borer pest?

Bt cotton
Bt rice
Golden rice
Hybrid maize
2

Bacillus thuringiensis (Bt) rice is genetically modified for resistance to the stem borer pest.

Which of the following is a correct statement regarding recombinant DNA technology?

Recombinant DNA technology involves the use of ribosomes to cut DNA.
Recombinant DNA is formed by joining DNA segments from different sources.
Recombinant DNA technology is used to produce hybrid seeds.
Recombinant DNA technology is only used in plant breeding.
2

Recombinant DNA technology involves combining DNA from different sources to form recombinant DNA for various applications, including medicine and agriculture.

Which of the following is correct regarding RNA interference (RNAi)?

RNAi enhances gene expression in cells.
RNAi silences specific gene expression using double-stranded RNA.
RNAi is used for DNA replication.
RNAi prevents cell division.
2

RNA interference (RNAi) silences specific gene expression using double-stranded RNA molecules.

Which of the following statements is correct about DNA ligase in genetic engineering?

DNA ligase is used to cut DNA at specific sites.
DNA ligase joins DNA fragments by forming phosphodiester bonds.
DNA ligase inhibits DNA replication in cells.
DNA ligase synthesizes mRNA from DNA.
2

DNA ligase is an enzyme used to join DNA fragments by forming phosphodiester bonds during recombinant DNA formation.

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